NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes

Techniques to stimulate and interrogate cell–cell adhesion mechanics

Cell–cell adhesions maintain the mechanical integrity of multicellular tissues and have recently been found to act as mechanotransducers, translating mechanical cues into biochemical signals. Mechanotransduction studies have primarily focused on focal adhesions, sites of cell-substrate attachment. These studies leverage technical advances in devices and systems interfacing with living cells thr...

Simultaneous high-resolution analysis of vaccinia virus and host cell transcriptomes by deep RNA sequencing.

Deep RNA sequencing was used to simultaneously analyze vaccinia virus (VACV) and HeLa cell transcriptomes at progressive times following infection. VACV, the prototypic member of the poxvirus family, replicates in the cytoplasm and contains a double-stranded DNA genome with approximately 200 closely spaced open reading frames (ORFs). The acquisition of a total of nearly 500 million short cDNA s...

Nuclear Transcriptomes at High Resolution Using Retooled INTACT.

Isolated nuclei provide access to early steps in gene regulation involving chromatin as well as transcript production and processing. Here, we describe transfer of the isolation of nuclei from tagged specific cell types (INTACT) to the monocot rice (Oryza sativa L.). The purification of biotinylated nuclei was redesigned by replacing the outer nuclear-envelope-targeting domain of the nuclear ta...

New high-efficiency protective coating containing glycidyl-POSS nanocage for improvement of solar cell electrical parameters

Various antireflection thin films are often used to cover glass to increase solar cell electrical parameters. In the recent years many efforts have been done to develop and improve of solar cell films with high electrical output. One of the most important challenges of obtaining of high-efficiency thin films of solar cells is creation an effective light trapping system. The new polymeric protec...

Implantable Microsystems for High-Resolution Interfacing to the Brain